inverted zeiss axio observer d1 microscope with a plan-apochromat 63x/1.40 oil dic objective  (Carl Zeiss)

Journal: NPJ Precision Oncology

Article Title: Optimized culturing yields high success rates and preserves molecular heterogeneity, enabling personalized screening for high-grade gliomas

doi: 10.1038/s41698-025-00946-1

Figure Lengend Snippet: a Brightfield images of four different representative glioma cultures (Zeiss Axio Observer D1 Inverted Phase Contrast Fluorescence Microscope, 10x magnification). All cultures show an astrocytoma-like phenotype with elongated cell shapes, slim protrusions, small cell bodies, and bright edges. Typically, unless a culture is severely overgrown, there is space between individual cell bodies. The size bar represents 100 μm. b Brightfield images (Zeiss Axio Observer D1 Inverted Phase Contrast Fluorescence Microscope, 10x magnification) of culture GS.0821 at passage 0 and at passage 6. At passage zero, a heterogeneous mix of two cell types can be observed: the astrocytoma-like phenotype and the fibroblast-like phenotype. c Immunofluorescent staining of neuronal stem cell marker SOX2 and GFAP (Leica TCS SP5 microscope). The black and white images show the positive cells for a single marker, the color image shows a composite image. d Percentage of marker-positive cells per arm of the protocol for cell culture GS.0875. Error bars represent differences between three different areas of the same cover slip. e Growth rates of the cultures from the four different arms of the protocol over three consecutive passages for three different tumors. Error bars represent the standard deviation between technical replicates.

Article Snippet: Bright field microscopy images were made on a routine basis with a Zeiss Axio Observer D1 Inverted Phase Contrast Fluorescence Microscope, HAL1 illuminator, and using a 10x Zeiss A-plan objective (421041-9910-000).

Techniques: Fluorescence, Microscopy, Staining, Marker, Cell Culture, Standard Deviation